NGS Course Reviews

Great explanations of the whole NGS process, with important advice about cost considerations, sampling, DNA extraction processes and the relevance of careful project/experiment planning. All of the instructors were easy to approach and extremely helpful/patient with answering a range of questions. I appreciate their time and expertise. The use of a comic cow always helps!

The course was facilitated by highly qualified people, with great ways to explain complex Linux programming. I really benefitted from the course and look forward to apply all my knowledge to my own work.

Amazing course! Very well organized and structured. Totally recommended

I thoroughly enjoyed following the course. The pace at which everything was introduced was very nice and I appreciated the background information on the different sequencing techniques.

Very interesting and informative course, after you want try and rework your data.

This is a great opportunity to learn the basics of computational analyses of NGS data and practice it real time. The overall arrangement, instructors and their methods, and the course structure - all are greatly satisfactory. It helped me to train my skills to start understanding the hows and whats of NGS data handling.

Overall I found the course very well organized and every need was met regarding our comfort and keeping us fed and hydrated which was much appreciated! The course itself was very well put together and extremely useful and informative. For someone with no previous experience, the practical Linux parts of learning the commands and execution was quite overwhelming and at times intimidating, however I'm very aware this will come with practice and takes time to grasp. Instructors were extremely approachable and open to questions. The materials provided were very useful and i look forward to playing around with the terminal myself. Overall would recommend to a friend!

The workshop was very informative for me and I would recommend it to my friends and colleagues who are interested.

Well, to be honest, I didn't know what to expect. I've gained a lot of experience in programming pipelines in Perl and Bash or Python over the last few years, I know the common bioinformatics tools, ... and yet I spent three full days learning so many new things that I now feel like a beginner again. And that's a, uhmm, good feeling :-) Adam led the workshop fantastically, with calm and patience. Even at 4:30 pm you still felt like listening and continuing. Always happy to do it again!

I liked the course a lot. It was well paced, with a nice mix of theory and practical exercises. Everything was easy to understand, and it helped that the examples were taken from basic bioinformatic concepts.

It was a great course covering the basics. A good starting point for anyone wanting to get into Single cell rnaseq work

It was an extremely condensed, organised and highly intellectual program. After the course, I felt comfortable with the material and the analysis of single cell RNA sequencing. I enjoyed every bit of the course, including the social aspect. I also appreciated that the tutors took their time to explain every question even when we were running short of time.

The course perfectly matched my needs. I have worked with already prepared integrated Seurat objects, and now I know how to do it on my own. Super nice atmosphere, great people, and many possibilities for networking. Not too fast and too slow.

Overall, I was extremely satisfied with this workshop. As a beginner to any sort of RNA analysis this was a great introduction and along with the course material book, I believe I could now tackle a dataset independently which is exactly what I wanted from this course. So, thank you all very much for organising such a superb workshop!

Excellent Workshop!

The course is well organized. The lecturers are very clear and always ready to give additional explanations. This course really helps those who need basic information on bioinformatics.

The workshop was excellent. I appreciated the teaching style and how they emphasized crucial details. Despite its brevity, spanning just three days, it was highly beneficial. So, Congratulations to the ecSeq Team once again!

This workshop taught me a lot on the NGS post-sequencing pipeline and the basics of Unix/Bash command-line. well organized, knowledgeable and helpful tutors.

A very comprehensive insight into the standard analysis workflow for processing NGS data just by using the Linux command line and common open source toolkits. I particularly liked the high practical component of the course as well as the access to the browser terminal, which allowed me to deepen the content discussed. The instructors have many years of expertise in their field, actively involve the participants in the discussion and fully address all questions. All in all, a very enjoyable course that I can highly recommend to anyone who wants to gain an understanding of bioinformatics analysis of NGS data.

Very informative workshop with well explained lectures and exercises. It covers different aspects of NGS, which are all needed to perform meaningful and high-quality data analysis. A great plus is that the virtual machine used in the course can be downloaded, so all tools can easily be used also afterwards, with one's own data (without the need to install them).

Fantastic course! The instructors demonstrated a deep understanding of each of the topics that were presented and a lot of useful detail was provided.

Really interesting training, with lots of interaction with the speakers.

I am writing to express my utmost satisfaction with the Bioanalysis in Single Cell Sequencing course. The comprehensive content covered every aspect needed, offering a full understanding of the topic. I would like to extend my gratitude to the exceptional instructors whose friendliness and eagerness to answer all our queries made the learning experience truly enriching. Their knowledge and commitment to our understanding were apparent and highly appreciated. I am looking forward to applying the skills and knowledge I have gained in my future work.

Outstanding workshop with competent trainers. I enjoyed the clear storyline, explanations and discussions. The content trained is very relevant to our daily in-house cross-functional project work.

Very clear and insightful training material greatly presented from both trainers with complementary expertises, enriching and pleasant discussions

I found the course difficult. I had no prior coding experience and even the German keyboard was very different from the version I am used to. I didn't attend the last two days as I did not think from the notes provided, I would learn much additional that I could not experiment by using the book at a later date. It has been a good way for me to learn a little about what bioinformatics is about. I would have liked to learn more about automation of workflows.

I really enjoyed the course. It helped me to further improve my knowledge about NGS. And learn a bit about using command line.

It was absolutely fantastic. Wonderfully organized and I learned so much.

The course was dense, well paced, but no stone was left unturned, particularly for statistics and "black boxes", which I particularly enjoyed. Organisation was very good, as well. The wit and expertise of the instructors made this the best such course I have so far attended.

It was a fun week full of learning. Adam and David were really helpful with the problems and the explanations.

Great course if you want to understand RNA-seq and you have no previous knowledge about the topic. The course gives you a helpful overview but also tools that you will be able to apply to your own data back home.

The knowledge I obtained during the workshop gave me the necessary tools to start using Nextflow in my Bioinformatic projects. I am satisfied and I would recommend it to anyone who wants to start developing with Nextflow.

Very interesting workshop.

For a 3-day course, it covers all aspects one needs to reflect on & start-up their own analysis. The explanations on quality control, alignment, mapping and eventually: variant calling, were enlightening. Each of these topics was covered piece by piece, with attention to questions and a limited background in bioinformatics. It gave us the opportunity get familiar with the Linux command-line, which was thoroughly explained and put to practice with exercises on template data.

Great course! It felt as a safe learning environment and help was readily available if needed.

What a learning curve, I really enjoyed the course! As I did not have any previous knowledge in programming, it was challenging at times, but the explanations by teachers and the course material helped a lot. Also the direct help in the Linux terminal was great. Regarding the theoretical parts of the course, I gained quite some knowledge and understanding on sequencing and data analysis, which will make it much easier for me to collaborate in our interdisciplinary project groups, and to learn more by myself in future.

The workshop gives a very compact overview to NGS data analysis

The instructors are professional, materials are practical

The workshop was very well organised, covering several relevant topics related to the analysis of unprocessed sequence data. The trainers were very well-informed on the subject-matter, and were able to answer every question asked during the workshop. I had a great learning experience and would highly recommend this course as a starting point for aspiring bioinformatitions.

The course was well organised and the instructors explained in great detail the theory behind each step of the RNA-seq analysis. A lot of time was also allocated in the practical part of the workshop, working on Linux and getting to know how commands are structured, and getting familiar with modifying commands and running scripts.

This hands-on nextflow workshop was very useful to get a better understanding of the language, from programming details to the larger picture of how to design and implement a new workflow. I liked that the transition to the DSL2-version of nextflow was also given attention. The presentation of the course material in the form of lectures was outstanding, while questions were welcomed any time and resolved quickly. The instructors were very helpful and patient whenever I ran into problems working on the exercises. All in all I learned a lot and feel lucky to have met the other participants, a group of very nice and friendly people from all over the world.

Another great course, with very pleasant and helpful trainers, as well as great international participants. In addition to the theoretical introduction to pipeline development, sufficient time is provided for practical exercises. The course materials provided allow a fast transfer of the course content into practice.

The workshop "Bioinformatics Pipeline Development with Nextflow" was a concise yet comprehensive introduction to this workflow management software that gets increasingly popular in bioinformatics.

The workshop exceeded my expectations. It was well designed and well laid out for 4 days. Apart from theory, there were excercises at each stage which worked well for basic as well as advanced users. The instructors were competent and their excellent communication helped us to absorb this advanced course. They also provided opportunity to discuss our custom problems.

The training was very good, useful and accessible. The team really took the time to guide us through the different steps of NGS analysis. The effort made to make this training understandable for people with limited IT skills was very appreciated. Trainers explained well the different principle and analysis tools. Overall the training gives a very nice introduction to start NGS analysis on its own.

An intensive and very interesting workshop! Learned a lot, especially about Linux. All the time we got great support from the ecSeq team.

The workshop was very useful, catering well to the intended audience. It was definitely a great introduction to NGS methods and data analysis, laying a good foundation for further learning and helped me get understanding on some things I was stuck with during my data analysis. The teachers were helpful with a sense of humor as well :)

Great summer school, very useful, I learnt a lot of concepts with a very high quality from expert people working on this field. Super!

Great workshop with interesting topics, good lectures and sufficient help when needed.

Training was excellent.

This was one of the most informative workshops I have ever attended, and would definitely recommend to my other colleagues who work on NGS platforms.

Great course to take when planning to start with nextflow pipelines. Provides a well-structured and founded introduction that allows understanding publicly available pipelines much better. Also writing first own nextflow pipelines definitely appears within reach after this course.

The course was intense but the trainers were fair and nice to take time to explain and help. It is not obvious for a biologist to perform such kind of analysis but I am glad I can pretend to at least try to understand how it works and dig in my own data in the future.

For me as a molecular biologist the course was too difficult as it was a lot of information and we went quite quick through the course. When you have no programming experience it is really difficult. I do like the Nextflow program and it is a very good course and I will try to invest time in the future to learn it step by step, guided by the information I have from the course.

This course was an excellent introduction to the biology and computational fundamentals needed to work with NGS data. I learned so much in 3 days and now feel confident to start working with the NGS data I've produced!

Very well organized and thought trough. As it is a practical course it is not easy to do it online, but with the people who helped in the back, it was well done.

Great course for those that are new to Linux and NGS data analysis. Step by step guidance with detailed explanations is provided. The course is well structured and the time is used efficiently.

Excellently structured and delivered course. It took you step by step through RNAseq right from the beginning with explanations of the experimental side to help understand the analysis side. The instructors were really knowledgeable and also made the course fun as well as educational. Would highly recommend!

I found this workshop was really useful and great, as it further extends my understanding on how to do the bioinformatics analysis on RNA-seq dataset. The workshop did not only teach you how to code to run the certain pipeline, but it demonstrated basic principles of how the pipeline works. Importantly, I also learned to evaluate my results and compare and contrast different pipeline tools for answering certain biological questions. it is therefore crucial, as for pure biologists, to understand the basic principles and skillsets on how to do the analysis, and in the future, they can adopt certain pipelines for their experimental data analysis. I proofed the Workshop was really useful because I finally managed to do differential expression analysis on my own sequencing data not so long after the workshop is done.

Very high quality quality courses with almost perfect pace and surprisingly good materials and forms of presenting it. The usage of virtual machine to keep everyone on a same page is a greate solution.

Outstanding

Great course to start your NGS analysis. Hands on experience with examples and support throughout the course.

I consider the course outstanding!

This course is amazing for anyone who is just entering the field of pipeline development or is a biologist who wants to know how to better streamline his NGS data analysis workflow.

Great workshop ! Having no experience with pipeline development, I thought I would have a harder time to follow than I actually did. The theory/practice balance was good. The technical difficulty is very approachable and thoughtfully adressed by good teachers. The skills I gained will definitely be applied to my work very soon.

This workshop provided a clear, basic and conceptual understanding of the Nextflow system. It is ideal as a first starting point for those who want to build and maintain pipelines written in Nextflow.

First-class coaching

It was a great course in NGS Data Analysis, with the correct amount of information and each step very well explained! It was very nice to meet all the people with the same problems and enjoy a bit Berlin ;)

The course provided an excellent introduction to the analysis of NGS data and covered the most relevant aspects in a well-structured and comprehensible way. The trainers did a really great job guiding us step-by-step through the NGS data analysis workflow and providing theoretical background information and lots of hands-on experience. Overall, a very informative and well-organised course - clear recommendation!

Very good and interesting training. Expanding my knowledge and skills. The third lecturer (Thursday and Friday) was really great.

Very good explanation of the entire pipeline and individual steps of NGS data analysis. The workshop improved my overall understanding of NGS analysis a lot, which also helps for future design of NGS experiments.

Very good. I will definitely recommend this course to others.

The course is very well structured and focuses all the main topics on NGS data analyses, with the appropriate detail for beginners. The tutors are excellent and very friendly and mentioned a lot of details, which only experienced users know. I have learnt a lot! Nevertheless, I found that the last day was very intense (or maybe I was just overwhelmed with the information from previous days).

A brilliant introduction to NGS analysis. Very clear explanations and a well thought-out structure.

The course provided a good introduction of NGS, covering the theory, practical issues and had a focus on the data analysis. For me personally, the last day of the course was not relevant, because I use NGS for phage display and the analysis was focussed on whole genome sequencing, including variant analysis, which I won't need. But everything was explained well for starters and the hands on experience using Linux was very useful.

Very good workshop, covers most of the basics of NGS data analysis that i was hoping for with nice, in-depth explanations for each commands and tools used.

I really enjoyed the nature of the workshop, I felt that everything was very approachable for the attendees to learn even if the experience was lacking. I witnessed some of my classmates who have no experience ask very detailed questions through the course of the workshop, so I think it is a testimonial to how informational the content of the workshop was.

I was blown away by the Course. I did some similar courses in the past and got little or nothing from them. This workshop gave me the ability to understand what is actually written in the command line, how to read it and the data and how to do the simple analysis myself. I am not terrified of the command line anymore. Actually, I can't get enough of it now. Also, I tried to reproduce the whole pipeline on my own data and actually got it all done using the knowledge and the resources from the workshop. All I need now is experince in reading the analysis :-D

It was an interesting course where I could learn a lot. It was not only interesting to see how nextflow itself works, but also very helpful to understand how such pipeline-tools work. Now I will be able to use such pipelines.

This is a fantastic course for beginners planning to self analyse NGS data. The course is well organised and explained in an easy common man language.

The handout, as well as the setting (terminal lock-in and zoom) was absolute in time and accurate. The frame of the workshop equips one with enough information to get started for the first NGS data set analysis. The four trainers were super friendly, open to help and most competent. The course is highly recommended.

It was a great course, well setup to work smoothly in a remote setting. The instructors were very knowledgable and managed to explain Linux commands and the steps of the NGS analysis pipeline in a simple easy-to-understand manner. The interactive exercises kept the group well engaged. I highly recommend to anyone wanting to get their feet wet in the world of bioinformatics.

(1) good Introduction, (2) hands on an practical, (3) technic worked quiet well, (4) good supervision with the shared terminal, (5) good material with stept by step (and really step by step) instruction

This course was excellent in obtaining updated tools for NGS analyses. Since the tools or applications change quickly over time, I felt up to date on how to proceed with my own projects. The course gave an excellent workflow or overview of NGS data processing from beginning to end. It was extremely convienent to take this class online.

Hello dear ecSeq team, the course was great! Perfect pace (it wasn't boring at any point but also not too fast), super understandable English, positive and infectious atmosphere, gave a super overview of the big picture. A huge compliment especially to David! The background help with programming was also always patient and helpful! Would recommend anytime!

This was a well done course, good job everyone! I really enjoyed it. The bioinformaticians were really good at explaining and guiding us with the NGS data. I especially liked the fact that we could have technical support with explanations directly on the Linux terminal, so we could immediately understand what mistakes were made while writing the various commands. I think this course is useful for people who have no experience with NGS data and Linux, but not only.

I enjoyed the workshop and felt like I was learning the necessary tools to start analyzing sequencing data. At the same time I did not feel like paying ~1000 EUR was justified.

I was really looking forward to your NGS Data Analysis Course and I was not disappointed! I could really dip in the big world of NGS and learned what to do with huge amounts of data and how to process them efficiently. The course offers a great view on NGS Data Analysis in only 3 days. Can definitely recommend!

An amazing workshop for anybody interested in an introduction to NGS data analysis!

I learned so much from this course. Thank you. I had previously had metagenomics and metataxonomics courses, which have not been enough for the depth and growing number of our gut microbiome studies. I have an IT background and this course was very well taught, I had no problem keeping up and I am sure this will help me grow even further in this direction.

The course is comprehensive covering both theory and hands-on practical aspects. It is an excellent course for beginner in the field of Bioinformatics.

To be honest, I am not good at online learning. However, the NGS Data Analysis workshop was well designed/organized that I even did not feel the difficulties being away from the instructors. I am quite happy to attend this workshop and I believe I have learned a lot.

The training was so informative and the practical part meets all expectations.

I found the course 'Next-Generation Sequencing Data Analysis: A Practical Introduction' excellent to improve my skills. I had several gaps in knowledge, which have been well addressed in the course. Starting from raw data we went step-by-step through variant calling and further interpretation. It is very intensive, but the way how it is organized allow beginners in this field to follow all steps even without any skills in Linux commands. The tutors are open to any questions and helpful in any steps required. I strongly recommend this course for anyone dealing with NGS data.

Excellently structured and polished workshop! The material was challenging but the way it was presented made it easy to follow and fun to engage with. The tutors made the learning environment really friendly and safe, and I got much more out of this workshop than I initially expected. Thank you!

Surprisingly I got sucked into the topic, which I thought would be very boring. I have the feeling I learned a lot and can understand the sequencing process much better. Thank you!

It is a very condensed course, covering really practical tricks for beginners on coding in Linux, and basically all the primary steps dealing with NGS data. Really worths the contribution of the fee and the time spent :)

Excellent workshop with outstanding teachers! I highly recommend it to anyone interested in Bioinformatics and/or NGS. Excellent organization in a great location. The invited talks were also outstanding.

An excellent course with just the right mixture of theoretical background, direct work in the command line and already preprepared intermetiate material to exucute certain analyses requiring more calculation time than available in a Course. I learnt a lot and this was really helpful.

The workshop was exactly like promised and I learned a lot. In fact, I am already regularly using the learned methods in my daily work.

Good course, but the Linux part was for me too big (experienced Linux user) although I still picked up something new.

This was an excellent course and I highly recommend it for anyone beginning to learn NGS data analysis. All of the topics were explained very well and it was the right amount of information for my first time taking a course on NGS data and using the Linux command line.

It was a good and easy to follow introduction into the topic and even though I had some prior experience with some of the tool presented it filled some knowledge gabs and will improve my work in the future.

It was a really good workshop with a high learning curve for me.

The instructors (Christian and Gero) were professional, authoritative and diligently attended all questions and doubts from the attendants. I was very new to the bioinformatics World and the 4 days were very intense, completely interactive and full of information. The course contents is very well curated, and pace & timing extremely well managed. Well done guys!

The content of the course met my expectations and needs as a new PI in the field of IBD immunogenetics. I very much appreciated the "behind scene" approach for few pipelines and the global overview of different approaches to correctly address the biological questions. I am very grateful to Christian and Gero who clearly demystified the RNAseq analysis part. I still need a lot of practice but I am very confident that I can do it now!

I very much benefited from the participation in this workshop. I liked the balance between pratical exercises and theoretical introduction into the subject. I always felt supported by the workshop leaders and my questions were answered sufficiently.

It was an awesome course!

This first workshop of NGS Introduction, is an excellent step for beginners in NGS data analysis and bioinformatics diagnostics. This technology transfered to us from experts with a very generous know-how and strong knowledges,the used tools are "open source". So, dont hesitate to get more familiar with "Algorithms"...

The workshop was very professional and of high quality. In very short time we went through linux basics to complex data evaluation. I am very satisfied.

The Next-Generation Sequencing data analysis introduction course gives a good overview about the technology itself and especially about the handling of raw data. It helps to understand Linux and how to use it for NGS data analysis.

The course gave a really deep and still concise overview of the basics of analysing bisulphite sequencing data. Good pedagogics skill from the very knowledgable course leaders made even the most complex features of the analyses comprehensible.

I would highly recommend this course to anyone beginning to work with NGS data. The practical aspect combined with the theory behind the analytical processes is a great starting point for interpreting your own data.

This workshop was an amazing introduction into NGS. I liked that the basics were well laid out and I now have a much clearer image of the entire workflow (it can appear quite literally opaque from the outside). The explanations on sequencing by synthesis, adapters and the way paired ends work were well laid out and put things into context for me as a wet lab researcher. I had used Linux for a number of years, but I was very surprised to learn that you can do almost everything by only basic programs that have been around since forever. As for the mapping tools, the stats & maths behind them are surely much deeper than I can comprehend, but you gave a good starting point - and of course each use case will have different optimal solutions. The genome viewer helped a lot, as well as explanations for the various file formats. I liked that the course was fully interactive and that participants were actively encouraged to ask questions. I will now try exploring RNAseq datasets of white blood cells (the domain that I work in) and see what I useful info I can come up with. I'd also like to delve into bisulfite sequencing (at least targeted) but that's more of a long shot for another project I work in.

A very good course that shows the background to how ngs data analysis is done. Some knowledge of Linux and programming will help a lot in the course.

Incredibly informative. Provided a great introduction to the programmes and can't wait to apply what we have learnt.

This course gave me a great introduction to the field of RNASeq analysis. It was well structured and conveyed all the information, which are required - except of some basic computer skills - to analyse your own data sets. Beside the practical skills, there was also a great focus on the theoretical background, which was well described by using simple examples. In summary, this course gave a nice overview of current RNA-Seq analysis methods and how results can be visualized. I can strongly recommend this course.

Excellent workshop!

Excellent!!!!!!!!!!!!

I was amazed by the excellent course structure, preparedness of the teachers, and practical relevance. The course allows in immediate start in NGS RNA-seq data analysis.

Great workshop. I learned alot.

This was by some distance the best bioinformatics workshop I have attended. It was great to consolidate everything I had previously learnt into a clear and concise framework. The tasks were challenging but manageable, and the three speakers were excellent. I would recommend this workshop to anyone with beginner/intermediate skills in sequencing analysis who wants to get more comfortable working in Linux and running sequencing pipelines.

Really good workshop, the information given to us was as much as you can learn in one week. Happy to had learn new info and techniques to analyze data.

Thanks for this great workshop. Even though I am no bioinformatician I learned a lot about the different steps of RNASeq data analysis. Not only the linux based commands but also the nice overview how the sequencing process itself works helped me a lot to get a deeper insight and understanding for library preparation and analysis.

The course was simply great! I was looking for an introduction to RNA seq analysis without need of prior experience and got exactly what I was looking for. The pace, the information and the structure of the course was perfect. It was a good mix of theory, going through exercises as a group and then trying it out for yourself to get a feel for it. I really learnt a lot, and the course leaders were extremely knowledgeable.

very interesting and important course for RNA-seq analysis

A very clear and functional introductory course to RNASeq data analysis. Very easy to follow, adequately presented and all course material was provided on site. A very nice touch was that they provided the possibility of taking home all digital files created during the course on a USB-drive.

I came with little practical knowledge of RNA-seq, and when I returned home, I was ready to analyze my own data!

Great introductive course! Very clear and informative, perfect to enter the world of NGS data analysis.

Very helpful, well organized. I loved the course.

I am a senior reseacher and my education basis is molcular biology. I am PI of several genetical including GWAS and human genome sequencing, but I have never performed the analyses myself. I wanted to attend the course to be able to guide analyses and to discuss the analysation part with the bioinformaticians. The course was exactly perfect for my intention and for my level. I really learned a lot and actually liked it! I can use a lot of this in my «everyday» activities, also in writing proposals etc., because I understand more about the work load and the lever of expertise needed.

The course was very well prepaired and good lead by Mario and David.

Well organized, focused,amiable learning environment

I was very positive surprised, how well the presenting and teaching of this Technology was. Overall the speed and the difficulty were very nice and helpful. The Organisation was well planned and the benefit that each of us, had a computer were very efficient for the direct excercise. So thanks a lot, I will definitely recommend this curse.

The course was very useful and entirely practically oriented.

This was a great workshop. Really well organized, great materials and outstanding teachers who presented the subject matter with clarity.

Positives:
1) I think both the lecturers were highly dedicated and very knowledgeable. 2) The course content was also very well documented. 3) All were very helpful and responsive to queries. 4) I think R and terminal commands were explained in a very efficient manner. 5) Lunch was well-organized.

Negatives:
1) The workshop was very fast paced from the beginner's point of view and packed with content. It would be ideal to either split it into two 4-day workshops or conduct a two-week course. 2) At times, some of the classes went on and on for 2.5 hours at a stretch. It doesn't make any sense for a candidate who has come to learn because one loses concentration after 40 min. 3) Please provide scripts in a R or txt file to students.

Very helpful from the practical point of view, clear explanation of every issue, experienced trainers that answered all my questions, great place, I loved it!

Very good workshop. The first two days contained information that I heard before, but it was really nice to hear it again, because it made me understand it better (Illumna, quality control). The last two days contained new information, like methylation calling and making plots, which is also very useful for my project. The workshop is intense, but the level and speed were just right. I would recommend new participants to gain a little bit of experience with Linux before attending the workshop.

The Workshop was very well designed. The description on the webpage was very good concurring with the content of the workshop and requirenments to the participants. As I did work through the Linux tutorial before, the first day was a bit boring for me, but a good practice at all. The second and third day I learned a lot of things. As I did not work with RNA-Seq before, the last day contained a bit a lot Information. The practical parts I could follow easily and the time was quite right so solve the tasks. The amount of paricipants felt ok.

Very satisfied though very condensed and intense course for a starter like me. Basic knowledge is recommended.

One of the best workshops I ever attended. The organization was just great. The small excercises during the workshop were quite useful to internalize the contents. It was fairly easy to follow even if one has not worked with linux before. I would strongly recommend the workshop!

Great experience, helpful and approachable instructors, interesting and well organized workshop. This course provided necessary training in NGS that can be used in research projects. Personally, being in the early stages of my undergraduate studies in Molecular Biology and Genetics this workshop really inspired me and expanded my horizons. More specifically, by meeting participants from all around the world who are involved in various fields of science, attending talks from external guest speakers who described their career path and getting to know the instructors of the course who were full of enthusiasm about Bioinformatics, I realized the interconnection between the scientific fields and the different professional options that I could potentially be involved with in the future.

The course was very well organised, the trainers were able to cater to the different needs of the participants, which had very different backgrounds. The atmosphere was relaxed, and the trainers made us feel comfortable to ask any question. The

An overall comprehensive and effective overview of the RNA-seq pipeline, from sample-prep considerations to high-level analysis and visualization.

I personally enjoyed the full course and it makes me very confident to do my future NGS analysis though I don't have a bioinformatics background. Everything was well organized and executed in a most efficient way for the participants. Simply, I can say that this course will make you gain confidence and extensive knowledge in the field of NGS bioinformatics. I loved it!!

This was a great workshop about different aspects of NGS data analysis focussing on putting analysis into practise. Motivated trainers with different expertises guided through the course excercises and in addition invited speakers gave a good idea about what NGS data might be useful for in different fields. Having some basic programming knowledge is certainly an advantage for following the practical parts.

The workshop was excellent. Speakers were very knowledgeable, ready and able to answer questions at all times. Great working material, with the workbook and the USB stick one can easily reproduce all the tasks.

Intensiver Kurs, extrem an Wissen gewonnen. Kompetente und motivierende "Trainer" und ein toller Gesamtüberblick. Das Kursbuch und besonders der LiveSystem USB-Stick sind super Toll und ermöglichen das weitere Üben, intensivieren des Gelernten.

Nice class for a comprehensive beginning of NGS data handling

Great workshop, intense and lots of invaluable information

This 'crash' course is a perfect course for quick guidance through analysis of RNA-seq and NGS analysis in general. Impressive how much new knowledge you can acquire in only 4 days!

I really enjoyed the course. It fulfilled my needs and definitely improved my understanding in bioinformatic tools and linux. Gaps I had are covered now. Compared to other courses I went to, in my opinion this is one of courses you are able to get started afterwards. All instructors are very kind and helpful :-).

It was really great performed training, prepared by experts in the field of bioinformatics. The course gave me opportunity to play with data from RNA and DNA sequencing by myself. Now I can't wait for my own results to analyze it.

Excellent workshop! Very well taken care of!! Great fun!!! Learned a lot!!!! and met very nice people!!!! After five days of smooth scrolling over a meticulous and interactive program one gets a good grasp of the intricancies of NGS data analysis. Many thanks!

Thanks to the very well designed and practical summer School for NGS data Analysis in Berlin I could find my feet and build up my mind in the early phase of my PhD project. I was looking for such course for a while and barely could find this opportunity to paractice NGS data analysis for both DNA and RNA approaches , having responseful mentors beside and a proper workstation available. I am very happy that I got this chance with ecSeq people.

This workshop was exactly what I was looking for - using publicly available software, comparing different tools and showing the whole process of data analysis. And I very much appreciate the idea of performing everything on the usb stick so that we can take everything we've done home and practice by ourselves.

It was one of the best introductory course I have had on the subject. The trainers go in to details of each and every nitty gritty of various topics. Also the exercises are designed very professionally and with clear learning objectives in mind. Finally, I learnt a lot many things during the discussions that are entertained very well by the trainers during the entire course.

Workshop is very useful for people without experience in bioinformatics to get basic understanding of NGS data analysis, and for those with some experience to improve their knowledge. Trainers are excellent.

After this workshop, I feel much more confident of my own bioinformatics skills. I can now dive into more specialized areas of bioinformatics, as I have a good basic knowledge of how everything works.

Being a novice to NGS data analysis this workshop was eye opening and made me confident in starting my own analyses.

The course was very informative and well organized. Everything was clearly explained and very helpful for beginners!

I was particularly impressed with the emphasis on teaching us how to THINK about our analyses, rather than just follow a series of commands. Your focus on anticipating potential sources of error, never blindly trusting that a command or piece of software has done what you think it's done without confirming (as well as teaching us ways to confirm) left us with a skill set that is independent of a particular pipeline, and will serve us well as our interests (and the available software) change. The active exercises were clearly designed extraordinarily carefully and were really effective not just in applying what we'd just learned, but in seeing how you might fall into an analysis/interpretation trap if you are not careful. This really was a mindset/philosophy of analysis training as much as it was practical training, and I value that enormously. Thank you!

The course was designed for people starting their work with RNA-Seq and was very well prepared and led. The information is provided in a clear and accessible manner and, most importantly, from a practical point of view. The trainers have extensive experience and provide valuable insights into the real problems arising from data analysis. I am happy to have had opportunity to attend these workshops. The knowledge and skills acquired during the workshops will be of great help in planning my own experiments and will enable me to start analyze the data on my own. I definately recommend these workshops to anyone planning RNA-seq experiments.

The workshop was really informative and conveyed a realistic overview of current RNA-Seq analysis. I particularly liked the emphasis on taking into account the experimental conditions and the goal of the study.

The workshop was well suited for beginners like me and was very relevant for my work. I think that what I learned will really help me to speed up analyses in the future.

A nice start point.

Very good week of course, very useful for future\ work on DNA methylation sequencing analysis.

ecSeq team pays a great attention to equip you to be on your own once back alone in your lab. They are recognised experts of the field and share not only their expertise but also their experience and views. Very valuable.

As someone who was previously terrified of the command line, this was a terrific course to build confidence in navigating the terminal. Moreover, I walked away with a much deeper understanding of sequencing chemistry and the relative merits of different software packages for analysing different types of sequencing data. I highly recommend this course for any wet lab biologist whether at the student, postdoc, or PI level!

The organisation of the Summer School was excellent.

There are too many things to consider when using NGS technology. I really needed a course like this to get started with a proper analysis of my own data.

This was a very intensive course, which gave tremendous help to anyone trying to work with NGS data. Difficult concepts were very effectively simplified and explained. Should be a great help in anyone interested in NGS.

The ecSeq workshop on "Next-Generation Sequencing Data Analysis" was very informative. I have no doubt that I acquired new skills, thus, extended my knowledge of analyzing the big data. I am integrating most of these in my current research analyses. Thank you ecSeq Bioinformatics Team!

This ECSEQ course provided a very good 3- day intensive workshop on NGS, Analysis, and BASH to give anyone working with genetic material a good foundation to expand their research project in a number of cutting edge directions. The instructors are warm and knowledgeable and eager to help anyone sincerely interested in learning these techniques.

A Beginner's Guide to RNA-Seq Data Analysis is an excellent workshop which provided me with the basic knowledge to start exploring RNA sequence data analysis and allowed me to address new questions. It includes an important component on very practical exercises, and the hands off material as well as the TI's and speakers are top quality. I highly recommend this workshop!

It was an excellent workshop. Thoroughly enjoyed the experience. It was very informative and the tutors were extremely helpful.

Since just having a little experience in analyzing RNA-Seq data, my knowledge about the unix environment, mapping tools and downstream analyses improved a lot during the workshop. With the help of the provided workshop materials it was quite easy to follow the excellent speakers. Several exercises supported the learning process as well.

To go from an introduction to methylation to the complete analysis of a published dataset in 3 days is an ambitious target and I am delighted that is was so comprehensively achieved. The mix between theory and practical work is very balanced. I highly recommend this course for a strong foundation in the latest methylation analysis methods. The course leaders are experts and so helpful, and to take the operating system, data and commands home at the end of the conference is a huge benefit.

Good resume of the last technologies and applications. Definitly will improve the comunication with our bioinformatics colleagues for perfom the proper analysis and understanding of the data. Thanks for an intensive but nice course.

Excellently prepared and performed workshop (from both organizing teams). A lot of information provided.

The ECSEQ RNA analysis course is one of the best courses. All the trainers were awesome. Many thanks to all of you.

Very good course, perfect for my needs.

Thanks a lot for a very interesting course that significantly improved my knowledge on NGS data analysis.

The workshop provided a great introduction into the analysis of NGS data for me as a wet lab biologist. The one day introduction into Linux was an absolute asset. Thanks to the course, I now feel comfortable both to discuss my NGS analysis needs with a bioinformatician more competently, but also to take first steps in NGS analyses myself.

As a wet lab biologist, I have been dabbling in analysing my RNA-seq data myself. This course gave me a lot of context about mapping and differential expression software that I hadn't been able to grasp on my own. This knowledge will certainly improve the quality of my analyses. Additionally, I learned a lot of handy Linux commands and tricks that make my life a lot easier.

Very good course, thanks for that.

Very efficient training in the basics of dealing with one of the most quickly evolving technologies we have.