9 - 13 March 2015
iad Pc-Pool, Rosa-Luxemburg-Straße 23, Leipzig, Germany
The purpose of this workshop is to get a deeper understanding in Next-Generation Sequencing (NGS) with a special focus on bioinformatics issues. Additionally, all workshop participants should be enabled to perform important tasks of NGS data analysis tasks themselves.
The first workshop module is an introduction to data analysis using Linux, assuring that all participants are able to follow the practical parts. The second module dicusses advantages and disadvantages of current sequencing technologies and their implications on data analysis. The most important NGS file formats (fastq, sam/bam, bigWig, etc.) are introduced and one proceeds with first hands-on analyses (QC, mapping, visualization). You will learn how to read and interprete QC plots, clip adapter sequences and/or trim bad quality read ends, get bioinformatics backgrounds about the read mapping and understand its problems (dynamic programming, alignment visualization, NGS mapping heuristics, etc.), perform your own mapping statistics and visualize your data in different ways (IGV, UCSC, etc.). The last two modules adress two specific applications of NGS: RNA-seq of model organisms and RNA-seq of non-model organisms.
The 2015 workshop has been redesigned and adapted to the needs of beginners in the field of NGS bioinformatics. The workshops comprises four course modules which can be combined.
|Linux for bioinformatics|
(industry: 300 EUR)
|Tuesday and Wednesday|
|Introduction to NGS data analysis|
(industry: 900 EUR)
|Thursday and Friday|
|RNA-seq Data Analyses|
|1. RNA-Seq of model organisms|
(industry: 750 EUR)
|2. RNA-Seq of non-model organisms|
(industry: 750 EUR)
This module is not available anymore.
*To attend this course, the "NGS Introduction" course has to be taken!
Gero Doose (University of Leipzig) found and published several circularized RNAs in various RNA-Seq experiments. He specialized on split-read analysis some years ago and has a strong expertise in downstream analyses.
Christian Otto (University of Leipzig) is one of the developers of the split-read mapping tool segemehl and is an expert on implementing efficient algorithms for HTS data analyses.
David Langenberger (ecSeq Bioinformatics) started working with small non-coding RNAs in 2006. Since 2009 he uses HTS technolgies to investigate these short regulatory RNAs as well as other targets. He has been part of several large HTS projects, for example the International Cancer Genome Consortium (ICGC).
Mario Fasold (ecSeq Bioinformatics) works in the analysis of microarray data since 2007 and developed several bioinformatics tools such as the Bioconductor package AffyRNADegradation and the Larpack program package. Since 2011 he specialized in the field of HTS data analysis and helped analysing sequecing data of several large consortium projects.
Opening Date of Registration: 10 November 2014
Closing Date of Early Registration: 15 January 2015
Closing Date of Registration: 1 March 2015
Workshop: 9 - 13 March 2015 (8:00 - 17:00)
Location: iad Pc-Pool, Rosa-Luxemburg-Straße 23, Leipzig, Germany
Available seats: 24 (first-come, first-served)
Travel expenses and accommodation are not covered by the registration fee.