Support

Welcome to our support page. It provides information for your everyday work with next-generation sequencing data as well as our tools and services. Frequently asked questions by our customers are answered here.

General NGS analysis

NGS Benchmark - comparison of different read mappers

Getting started with NGS data analysis: An overview

Important file formats for NGS data analysis

Convert BCL to FASTQ files in Windows

What is a good sequencing depth for bulk RNA-Seq?

Is it possible to select any insert for Illumina sequencing?

Why do Illumina reads all have the same length when sequencing differently sized fragments?

Subject specific terms and wordings for NGS

How to calculate the coverage for a NGS experiment

How are base qualities calculated and stored?

How do strand specific sequencing protocols work?

Best RNA-Seq aligner: A comparison of mapping tools

Are there regions in the genome that are not covered by DNA sequencing?

Is there a bias after DNA fragmentation?

How can unique molecular identifiers (UMIs) help to reduce quantitative biases?

What is mate pair sequencing for?

Do you have two colors or four colors in Illumina?

Why does the per base sequence quality decrease over the read in Illumina?

How to analyze NGS data: An overview of nine different IT solutions

Trimming adapter sequences - is it necessary?

Convert nucleotide sequences with IUPAC codes to an regular expression

What is the best NGS alignment software?

Epigenetics

How does bisulfite sequencing (WGBS/RRBS) work?

Code snippets for sequencing data analysis

Convert an interleaved fasta file to a single line fasta using only the Linux command line

How to get a coverage graph in WIG file format directly from an alignment (BAM)

Convert FASTQ to FASTA on the command line

Annotate mapping entries of a BAM file based on overlaps with BED files using BEDtools

Convert BAM to BED file format using command line perl

Add gzip/gunzip support to a program that doesn't have it (e.g. bowtie)

Extract a list of specific read IDs from a bam file

Run time-consuming processes in parallel on Unix systems

Support the next-generation sequencing knowledge bank

Recent posts

Getting started with NGS data analysis: An overview

Important file formats for NGS data analysis

Convert BCL to FASTQ files in Windows

General NGS analysis

NGS Benchmark - comparison of different read mappers

Getting started with NGS data analysis: An overview

Important file formats for NGS data analysis

Convert BCL to FASTQ files in Windows

What is a good sequencing depth for bulk RNA-Seq?

Is it possible to select any insert for Illumina sequencing?

Why do Illumina reads all have the same length when sequencing differently sized fragments?

Subject specific terms and wordings for NGS

How to calculate the coverage for a NGS experiment

How are base qualities calculated and stored?

How do strand specific sequencing protocols work?

Best RNA-Seq aligner: A comparison of mapping tools

Are there regions in the genome that are not covered by DNA sequencing?

Is there a bias after DNA fragmentation?

How can unique molecular identifiers (UMIs) help to reduce quantitative biases?

What is mate pair sequencing for?

Do you have two colors or four colors in Illumina?

Why does the per base sequence quality decrease over the read in Illumina?

How to analyze NGS data: An overview of nine different IT solutions

Trimming adapter sequences - is it necessary?

Convert nucleotide sequences with IUPAC codes to an regular expression

What is the best NGS alignment software?

Epigenetics

How does bisulfite sequencing (WGBS/RRBS) work?

Code snippets for sequencing data analysis

Convert an interleaved fasta file to a single line fasta using only the Linux command line

How to get a coverage graph in WIG file format directly from an alignment (BAM)

Convert FASTQ to FASTA on the command line

Annotate mapping entries of a BAM file based on overlaps with BED files using BEDtools

Convert BAM to BED file format using command line perl

Add gzip/gunzip support to a program that doesn't have it (e.g. bowtie)

Extract a list of specific read IDs from a bam file

Run time-consuming processes in parallel on Unix systems